|
 Academic Year:
| 2012/3 |
| Owning Department/School:
| Department of Biology & Biochemistry |
| Credits:
| 6 |
| Level:
| Certificate (FHEQ level 4) |
| Period: |
Semester 2 |
| Assessment:
| EX 70%, MC 10%, PR 20% |
| Supplementary Assessment: | Like-for-like reassessment (where allowed by programme regulations) |
| Requisites:
| Before taking this unit you must take BB10003 and take CH10088 and while taking this unit you must take BB10004 and take CH10087 |
| Description:
| Aims: To provide understanding of the principles and applications of various methods for the purification and characterisation of proteins. Learning Outcomes: After taking this unit the student should be able to: * Describe common methods for purifying proteins and be able to design a simple protein purification scheme; * Describe the use of different spectroscopic techniques to probe structural features of proteins; * Describe physical techniques used to elucidate the function and oligomeric state of proteins. Skills: Learning and studying T/F/A, Written communication T/F/A, Numeracy & computation T/F/A, Laboratory skills T/F, Information technology T/F, Information handling & retrieval T/F/A, Working independently T/F. Content: Protein separation by column chromatography based on differences in molecular size, charge, hydrophobicity and specific affinity for ligands; electrophoretic methods for the purification and characterisation of proteins; use of techniques (such as fluorescence, UV/CD spectroscopy, dynamic light scattering, mass spectroscopy, ITC and SPR) to probe function and oligomeric state of proteins. |
| Programme availability: |
BB10186 is Compulsory on the following programmes:Department of Biology & Biochemistry
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