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BB20144: Techniques in cell biology

Follow this link for further information on academic years Academic Year: 2012/3
Follow this link for further information on owning departmentsOwning Department/School: Department of Biology & Biochemistry
Follow this link for further information on credits Credits: 6
Follow this link for further information on unit levels Level: Intermediate (FHEQ level 5)
Follow this link for further information on period slots Period: Semester 1
Follow this link for further information on unit assessment Assessment: EX 70%, PR 30%
Follow this link for further information on supplementary assessment Supplementary Assessment: Like-for-like reassessment (where allowed by programme regulations)
Follow this link for further information on unit rules Requisites: Before taking this unit you must take BB10005 and while taking this unit you must take BB20024
Follow this link for further information on unit content Description: Aims:
To give an overview of some of the commonly used techniques in cell biology and to provide an understanding of how these techniques are being applied to address current cell biological problems.

Learning Outcomes:
After taking this course the student should be able to:
* Describe and explain the basic principles involved in each technique;
* Determine and explain when a technique should be used;
* Assess the strengths and weaknesses of each technique;
* Plan their time during the course of an experiment for maximal efficiency and optimal outcome.

Skills:
Learning and studying T/F/A, Written communication T/F/A, Laboratory skills T/F, Information technology T/F, Problem solving T/F, Information handling & retrieval T/F/A, Working independently T/F, Career preparation T/F.

Content:
Lectures will cover the basic principles underlying the techniques of light and electron microscopy including immunofluorescence and confocal microscopy, immunoblotting, immunoprecipitation, cell culture, transfection of mammalian cell lines including considerations of types of promoters and cell type specific expression, subcellular fractionation and metabolic labelling. The practical content will include projects focused on transfection of mammalian cell lines, immunofluroescence, immunoblottting, and subcellular fractionation.
Follow this link for further information on programme availabilityProgramme availability:

BB20144 is Compulsory on the following programmes:

Department of Biology & Biochemistry
  • USBB-AFB05 : BSc (hons) Molecular and Cellular Biology (Full-time) - Year 2
  • USBB-AKB08 : BSc (hons) Molecular and Cellular Biology with Professional Placement (Full-time with Thick Sandwich Placement) - Year 2

Notes:
* This unit catalogue is applicable for the 2012/13 academic year only. Students continuing their studies into 2013/14 and beyond should not assume that this unit will be available in future years in the format displayed here for 2012/13.
* Programmes and units are subject to change at any time, in accordance with normal University procedures.
* Availability of units will be subject to constraints such as staff availability, minimum and maximum group sizes, and timetabling factors as well as a student's ability to meet any pre-requisite rules.