- Student Records
Programme & Unit Catalogues


BB10186: Proteins: purification and characterisation

Follow this link for further information on academic years Academic Year: 2018/9
Further information on owning departmentsOwning Department/School: Department of Biology & Biochemistry
Further information on credits Credits: 6      [equivalent to 12 CATS credits]
Further information on notional study hours Notional Study Hours: 120
Further information on unit levels Level: Certificate (FHEQ level 4)
Further information on teaching periods Period:
Semester 2
Further information on unit assessment Assessment Summary: EX 75%, MC 25%
Further information on unit assessment Assessment Detail:
  • MCQ (MC 25%)
  • Exam (EX 75%)
Further information on supplementary assessment Supplementary Assessment:
Like-for-like reassessment (where allowed by programme regulations)
Further information on requisites Requisites: Before taking this module you must take BB10003 AND take CH10088
While taking this module you must take BB10121 AND take CH10087
Further information on descriptions Description: Aims:
To provide understanding of the principles and applications of various methods for the purification and characterisation of proteins.

Learning Outcomes:
After taking this unit the student should be able to:
* Describe common methods for purifying proteins and be able to design a simple protein purification scheme;
* Describe the use of different spectroscopic techniques to probe structural features of proteins;
* Describe physical techniques used to elucidate the function and oligomeric state of proteins.

Skills:
Learning and studying T/F/A, Written communication T/F/A, Numeracy & computation T/F/A, Laboratory skills T/F, Information technology T/F, Information handling & retrieval T/F/A, Working independently T/F.

Content:
Protein separation by column chromatography based on differences in molecular size, charge, hydrophobicity and specific affinity for ligands; electrophoretic methods for the purification and characterisation of proteins; use of techniques (such as fluorescence, UV/CD spectroscopy, dynamic light scattering, mass spectroscopy, ITC and SPR) to probe function and oligomeric state of proteins.
Before taking this unit you must take BB10003 and take CH10088 and while taking this unit you must take BB10004 and take CH10087
Further information on programme availabilityProgramme availability:

BB10186 is Compulsory on the following programmes:

Department of Biology & Biochemistry

Notes: