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Transmission Electron Microscopy for Biochemists

November 13th & 15th 2009

Course lecturers: Ursula Potter and Hugh Perrott

This course takes place in semester 1 and comprises three lectures. It is run in conjunction with the Department of Biology & Biochemistry for second year undergraduate students in Biochemistry. It focuses on transmission electron microscopy and the specialised techniques used to locate and/or visualize cell components (proteins, nucleic acids, carbohydrates). For example: Immunocytochemistry, in situ hybridisation, cryo-transmission electron microscopy and 3-D protein and virus reconstruction.

- Lecture 1
Optical versus electron microscopy
- Lecture 2
Techniques for preparing biological tissue for TEM
- Lecture 3
Selective labelling & 3-D reconstruction of cell components

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