Department of Pharmacy and Pharmacology

albert_bolhuis

Senior Lecturer

7 West - 2.10

E-mail: A.Bolhuis@bath.ac.uk

Tel: +44 (0) 1225 383813 

 

Dr Albert Bolhuis 

Profile

Research Interests

Prokaryotes transport large numbers of proteins that end up either on the cell surface or in the extracellular environment. These proteins play various roles in for instance the uptake of nutrients or virulence. Investigating these proteins and how they are secreted is a long-standing research theme of the group. In this we have a particular focus on Gram-positive organisms such as enterococci. The latter have emerged as opportunistic pathogens and are a leading cause of hospital-acquired infections. Enterococci are very tolerant to various adverse conditions. Moreover, many strains are also able to form biofilms, which are communities of cells that adhere to surfaces. Biofilms are more difficult to clear by our immune system and are also very resistant to antibiotics, which is why this is clinically very relevant. Biofilm formation, and in particular the role of surface structures in this process, is currently one of our main interests.

Another line of research is on antimicrobials. Antibiotic resistance is a major problem in healthcare, and it is thus very important to continuously look for novel compounds to combat pathogenic bacteria. Ideally, compounds are found to which bacteria do not become resistant. To that purpose, various strategies can be followed. In collaboration with chemists we have analysed various DNA-binding compounds, with the idea that at least it will be difficult for bacteria to develop resistance against DNA by altering its target. A number of these DNA-binding compounds have indeed antimicrobial activity and using the simple nematode infection model we have shown that some of the compounds are able to rescue the nematodes from infection with MRSA. That indicates not only that these compounds are active in vivo, but also that they are not particularly toxic to nematodes.

Our research is funded by the BBSRC and the University of Bath.

Publications

Nguyen, P. T. M., Schultze, N., Boger, C., Alresley, Z., Bolhuis, A. and Lindequist, U., 2017. Anticaries and antimicrobial activities of methanolic extract from leaves of Cleistocalyx operculatus L. Asian Pacific Journal of Tropical Biomedicine, 7 (1), pp. 43-48.

Alhusein, N., Scott, J., Kasprzyk-Hordern, B. and Bolhuis, A., 2016. Development of a filter to prevent infections with spore-forming bacteria in injecting drug users. Harm Reduction Journal, 13 (1), 33.

Alhusein, N., Blagbrough, I. S., Beeton, M. L., Bolhuis, A. and De Bank, P. A., 2016. Electrospun Zein/PCL Fibrous Matrices Release Tetracycline in a Controlled Manner, Killing Staphylococcus aureus Both in Biofilms and Ex Vivo on Pig Skin, and are Compatible with Human Skin Cells. Pharmaceutical Research, 33 (1), pp. 237-246.

Kortman, G. A. M., Mulder, M. L. M., Richters, T. J. W., Shanmugam, N. K. N., Trebicka, E., Boekhorst, J., Timmerman, H. M., Roelofs, R., Wiegerinck, E. T., Laarakkers, C. M., Swinkels, D. W., Bolhuis, A., Cherayil, B. J. and Tjalsma, H., 2015. Low dietary iron intake restrains the intestinal inflammatory response and pathology of enteric infection by food-borne bacterial pathogens. European Journal of Immunology, 45 (9), pp. 2553-2567.

Alhusein, N., Blagbrough, I., Bolhuis, A. and De Bank, P., 2015. Controlled Release of Tetracycline From Electrospun Micro/Nanofibrous Zein/PCL Blended Mats: In Vitro and Ex Vivo Testing. In: Controlled Release Society Annual Meeting, 2015-07-26 - 2015-07-29.

Peck, O. P. W., Chew, Y. M. J., Bird, M. R. and Bolhuis, A., 2015. Application of fluid dynamic gauging in the characterization and removal of biofouling deposits. Heat Transfer Engineering, 36 (7-8), pp. 685-694.

Beeton, M., Aldrich-Wright, J.R. and Bolhuis, A., 2014. The antimicrobial and antibiofilm activities of copper(II) complexes. Journal of Inorganic Biochemistry, 140, pp. 167-172.

Alhusein, N., De Bank, P., Blagbrough, I. and Bolhuis, A., 2014. Controlled release of tetracycline hydrochloride from electrospun micro/nanofibrous mats: in vitro and ex vivo testing. In: PharmSci APSGB Meeting, 2011-01-26.

Bolhuis, A. and Aldrich-Wright, J. R., 2014. DNA as a target for antimicrobials. Bioorganic Chemistry, 55, pp. 51-59.

Peck, O., Chew, J., Bird, M. and Bolhuis, A., 2014. An assessment of the removal behaviour of biofilms grown under static and flow conditions.

Alhusein, N., De Bank, P. A., Blagbrough, I. S. and Bolhuis, A., 2013. Killing bacteria within biofilms by sustained release of tetracycline from triple-layered electrospun micro/nanofibre matrices of polycaprolactone and poly(ethylene-co-vinyl acetate). Drug Delivery and Translational Research, 3 (6), pp. 531-541.

Alhusein, N., De Bank, P., Blagbrough, I. and Bolhuis, A., 2013. Antibiotic Electrospun Micro/Nanofibrous Wound Dressing Formulations - In Vitro Testing Against Biofilms. In: PharmSci APSGB Meeting, 2011-01-26.

Peck, O., Chew, J., Bird, M. and Bolhuis, A., 2013. Application of fluid dynamic gauging in the characterisation and removal of biofouling deposits.

Krause-Heuer, A.M., Leverett, P., Aldrich-Wright, J.R. and Bolhuis, A., 2012. Copper(ii) and palladium(ii) complexes with cytotoxic and antibacterial activity. Australian Journal of Chemistry, 65 (7), pp. 860-873.

Howson, S. E., Bolhuis, A., Brabec, V., Clarkson, G. J., Malina, J., Rodger, A. and Scott, P., 2012. Optically pure, water-stable metallo-helical ‘flexicate’ assemblies with antibiotic activity. Nature Chemistry, 4 (1), pp. 31-36.

Akkaya, Ö., İşsever Öztürk, S., Bolhuis, A. and Gümüşel, F., 2012. Mutations in the translation initiation region of the pac gene resulting in increased levels of activity of penicillin G acylase. World Journal of Microbiology and Biotechnology, 28 (5), pp. 2159-2164.

Boleij, A., Muytjens, C. M. J., Bukhari, S. I., Cayet, N., Glaser, P., Hermans, P. W. M., Swinkels, D. W., Bolhuis, A. and Tjalsma, H., 2011. Novel clues on the specific association of Streptococcus gallolyticus subsp gallolyticus with colorectal cancer. Journal of Infectious Diseases, 203 (8), pp. 1101-1109.

Bolhuis, A., Hand, L., Marshall, J. E., Richards, A. D., Rodger, A. and Aldrich-Wright, J., 2011. Antimicrobial activity of ruthenium-based intercalators. European Journal of Pharmaceutical Sciences, 42 (4), pp. 313-317.

Bolhuis, A. and Richards, A. D., 2011. Development of metal complexes as potential antimicrobials. In: Aldrich-Wright, J., ed. Metallointercalators: Synthesis and Techniques to Probe Their Interactions with Biomolecules. New York: Springer, pp. 373-388.

Fasehee, H., Westers, H., Bolhuis, A., Antelmann, H., Hecker, M., Quax, W. J., Mirlohi, A. F., van Dijl, J. M. and Ahmadian, G., 2011. Functional analysis of the sortase YhcS in Bacillus subtilis. Proteomics, 11 (19), pp. 3905-3913.

Kwan, D. and Bolhuis, A., 2010. Analysis of the twin-arginine motif of a haloarchaeal Tat substrate. FEMS Microbiology Letters, 308 (2), pp. 138-143.

Richards, A. D., Rodger, A., Hannon, M. J. and Bolhuis, A., 2009. Antimicrobial activity of an iron triple helicate. International Journal of Antimicrobial Agents, 33 (5), pp. 469-472.

Meredith, K., Bolhuis, A. and O’Neill, M. A. A., 2009. Enterococcal surface protein Esp affects antibiotic sensitivity in Enterococcus faecium. International Journal of Antimicrobial Agents, 34 (4), pp. 392-393.

Kwan, D. C., Thomas, J. R. and Bolhuis, A., 2008. Bioenergetic requirements of a Tat-dependent substrate in the halophilic archaeon Haloarcula hispanica. FEBS Journal, 275 (24), pp. 6159-6167.

Bolhuis, A., Kwan, D. and Thomas, J. R., 2008. Halophilic adaptation of proteins. In: Siddiqui, K. S. and Thomas, T., eds. Protein Adaptation in Extremophiles. New York: Nova Science, pp. 71-104.

Bolhuis, A., Kwan, D. and Thomas, J. R., 2008. Halophilic adaptations of proteins. In: Siddiqui, K. S., Thomas, T. and Uversky, V., eds. Protein Adaptation in Extremophiles. New York, U. S. A.: Nova Science, pp. 71-104.

This list was generated on Mon Oct 23 11:22:30 2017 IST.

View more publications »