Department of Pharmacy and Pharmacology
mark_lindsay
Professor in Molecular Pharmacology

7 West 3.14

Email: m.a.lindsay@bath.ac.uk

Tel: +44 (0) 1225 386783

 

Prof Mark Lindsay

Profile

Upon completion of the human genome project, the biggest surprise was that humans have far fewer genes than previously expected (~ 21,000). Additionally, less than 2% of the available DNA directly codes for these genes. To address this paradox, recent studies have suggested that much of the remaining 98% of DNA is turned into 'non-coding RNA' and that these regulate the expression of gene expression at the level of transcription and translation. For convenience, we commonly divide this non-coding RNA into small non-coding RNAs and long non-coding RNAs. At the present time, we have little idea about the role of the majority of this non-coding RNA.

My research group is interested in using oligonucleotide-based therapeutics (siRNA and antisense) to target the down-regulation or inhibition of protein coding mRNAs and non-coding RNAs. In the last few years, the emphasis has been upon using next generation sequencing to identify mRNAs, miRNAs and long non-coding RNAs (lncRNAs) that underlying biological responses/diseases.

Current project include:

  • The role of long non-coding RNAs and the innate immune response (James Heward and Dr Benoir Roux)
  • Transcriptome analysis in re-current squamous cell lung cancer (Dr Andre Koper - Manchester)
  • Transcriptome analysis in muscle myositis (Dr Philip Hamann)
  • Age Related Changes in miRNAs expression (Jasmine Malchrist)
  • Models of rhinovirus infection in human lungs (Anna Pomerenke - Manchester)
  • Identification of genetic and serum biomarkers of psoriatic arthritis (Dr Deepak Jadon)

Collaborations include:

Professor Fan Chung (Imperial College) – Transcriptome analysis in airway smooth muscle in normal, mild and severe asthma

Dr Simon Jones (University of Birmingham) - Examination of the transcriptome in the synovial fibroblasts of patients with osteoarthritis (normal weight versus obese individuals)

Publications

Jadon, D. R., Sengupta, R., Nightingale, A., Lu, H., Dunphy, J., Green, A., Elder, J. T., Nair, R. P., Korendowych, E., Lindsay, M. A. and McHugh, N. J., 2017. Serum bone-turnover biomarkers are associated with the occurrence of peripheral and axial arthritis in psoriatic disease:a prospective cross-sectional comparative study. Arthritis Research & Therapy, 19 (1), p. 210.

Lindsay, M., Roux, B., Donnelly, L., Heward, J. and Jones, S., 2017. Catalog of Differentially Expressed Long Non-Coding RNA following Activation of Human and Mouse Innate Immune Response. Frontiers in Immunology, 8, 1038.

Hamann, P. D., Roux, B. T., Heward, J. A., Love, S., McHugh, N. J., Jones, S. W. and Lindsay, M. A., 2017. Transcriptional profiling identifies differential expression of long non-coding RNAs in Jo-1 associated and inclusion body myositis. Scientific Reports, 7 (1), 8024.

Austin, P. J., Tsitsiou, E., Boardman, C., Jones, S. W., Lindsay, M. A., Adcock, I. M., Chung, K. F. and Perry, M. M., 2017. Transcriptional profiling identifies the long noncoding RNA plasmacytoma variant translocation (PVT1) as a novel regulator of the asthmatic phenotype in human airway smooth muscle. Journal of Allergy and Clinical Immunology, 139 (3), pp. 780-789.

Moschos, S. A., Usher, L. and Lindsay, M. A., 2017. Clinical potential of oligonucleotide-based therapeutics in the respiratory system. Pharmacology and Therapeutics, 169, pp. 83-103.

Koper, A., Zeef, L. A. H., Joseph, L., Kerr, K., Gosney, J., Lindsay, M. A. and Booton, R., 2017. Whole transcriptome analysis of preinvasive and invasive early squamous lung carcinoma in archival laser microdissected samples. Respiratory Research, 18 (1), 12.

Jadon, D. R., Sengupta, R., Nightingale, A., Lindsay, M., Korendowych, E., Robinson, G., Jobling, A., Shaddick, G., Bi, J., Winchester, R., Giles, J. T. and McHugh, N. J., 2016. Axial Disease in Psoriatic Arthritis study:defining the clinical and radiographic phenotype of psoriatic spondyloarthritis. Annals of the Rheumatic Diseases

Pomerenke, A., Lea, S., Herrick, S. E., Lindsay, M. and Singh, D., 2016. Characterization of TLR-induced inflammatory responses in COPD and control lung tissue explants. International Journal of Chronic Obstructive Pulmonary Disease OPD, 11 (1), pp. 2409-2417.

Pearson, M. J., Philp, A. M., Heward, J. A., Roux, B. T., Walsh, D. A., Davis, E. T., Lindsay, M. and Jones, S. W., 2016. Long intergenic non-coding RNAs mediate the human chondrocyte inflammatory response and are differentially expressed in osteoarthritis cartilage. Arthritis & Rheumatology, 68 (4), pp. 845-856.

Ngkelo, X. A., Hoffmann, R. F., Durham, A. L., Marwick, J. A., Brandenburg, S. M., De Bruin, H. G., Jonker, M. R., Rossios, C., Tsitsiou, E., Caramori, G., Contoli, M., Casolari, P., Monaco, F., Andò, F., Speciale, G., Kilty, I., Chung, K. F., Papi, A., Lindsay, M. A., Ten Hacken, N. H. T., Van Den Berge, M., Timens, W., Barnes, P. J., Van Oosterhout, A. J., Adcock, I. M., Kirkham, P. A. and Heijink, I. H., 2015. Glycogen synthase kinase-3β modulation of glucocorticoid responsiveness in COPD. American Journal of Physiology-Lung Cellular and Molecular Physiology, 309 (10), L1112-L1123.

Roux, B. T. and Lindsay, M. A., 2015. LincRNA signatures in human lymphocytes. Nature Immunology, 16 (3), pp. 220-222.

Heward, J. A., Roux, B. T. and Lindsay, M. A., 2015. Divergent signalling pathways regulate lipopolysaccharide-induced eRNA expression in human monocytic THP1 cells. FEBS Letters, 589 (3), pp. 396-406.

Jadon, D. R., Nightingale, A. L., McHugh, N. J., Lindsay, M. A., Korendowych, E. and Sengupta, R., 2015. Serum Soluble Bone Turnover Biomarkers in Psoriatic Arthritis and Psoriatic Spondyloarthropathy. The Journal of Rheumatology, 42 (1), pp. 21-30.

Jolly, P., Wong, L. C. C., Miodek, A., Lindsay, M. and Estrela, P., 2015. A simple and highly sensitive electrochemical platform for detection of microRNAs. In: 2015 IEEE Sensors, 2015-11-01 - 2015-11-04. IEEE, pp. 803-806.

Heward, J.A. and Lindsay, M.A., 2014. Long non-coding RNAs in the regulation of the immune response. Trends in Immunology, 35 (9), pp. 408-419.

Booton, R. and Lindsay, M.A., 2014. Emerging role of microRNAs and long noncoding RNAs in respiratory disease. Chest, 146 (1), pp. 193-204.

Ilott, N. E., Heward, J. A., Roux, B., Tsitsiou, E., Fenwick, P. S., Lenzi, L., Goodhead, I., Hertz-Fowler, C., Heger, A., Hall, N., Donnelly, L. E., Sims, D. and Lindsay, M. A., 2014. Long non-coding RNAs and enhancer RNAs regulate the lipopolysaccharide- induced inflammatory response in human monocytes. Nature Communications, 5, 3979.

Perry, M.M., Tsitsiou, E., Austin, P.J., Lindsay, M.A., Gibeon, D.S., Adcock, I.M. and Chung, K.F., 2014. Role of non-coding RNAs in maintaining primary airway smooth muscle cells. Respiratory Research, 15 (1), 1 - 12.

Kankaanranta, H., Ilmarinen, P., Zhang, X., Adcock, I.M., Lahti, A., Barnes, P.J., Giembycz, M.A., Lindsay, M. A. and Moilanen, E., 2014. Tumour necrosis factor-α regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-κB and AP-1. PLoS ONE, 9 (2), e90298.

Tsitsiou, E., Williams, A. E., Moschos, S. A., Patel, K., Rossios, C., Jiang, X., Adams, O. D., MacEdo, P., Booton, R., Gibeon, D., Chung, K. F. and Lindsay, M. A., 2012. Transcriptome analysis shows activation of circulating CD8+ T cells in patients with severe asthma. Journal of Allergy and Clinical Immunology, 129 (1), pp. 95-103.

Brown, L., Joseph, L., Crosbie, P., Landberg, G., Barber, P., Lindsay, M. and Booton, R., 2012. RNA from FFPE tissue is suitable for exon array analysis if extracted utilizing a robust protocol which incorporates laser capture microdissection. Lung Cancer, 75 (Supplement 1), S4.

Jiang, X., Tsitsiou, E., Herrick, S. E. and Lindsay, M. A., 2010. MicroRNAs and the regulation of fibrosis. FEBS Journal, 277 (9), pp. 2015-2021.

Larner-Svensson, H. M., Williams, A. E., Tsitsiou, E., Perry, M. M., Jiang, X., Chung, K. F. and Lindsay, M., 2010. Pharmacological studies of the mechanism and function of interleukin-1beta-induced miRNA-146a expression in primary human airway smooth muscle. Respiratory Research, 11 (1), 68.

Perry, M. M., Williams, A. E., Tsitsiou, E., Larner-Svensson, H. M. and Lindsay, M. A., 2009. Divergent intracellular pathways regulate interleukin-1β-induced miR-146a and miR-146b expression and chemokine release in human alveolar epithelial cells. FEBS Letters, 583 (20), pp. 3349-3355.

Williams, A. E., Larner-Svensson, H., Perry, M. M., Campbell, G. A., Herrick, S. E., Adcock, I. M., Erjefalt, J. S., Chung, K. F. and Lindsay, M. A., 2009. MicroRNA expression profiling in mild asthmatic human airways and effect of corticosteroid therapy. PLoS ONE, 4 (6), e5889.

Tsitsiou, E. and Lindsay, M. A., 2009. microRNAs and the immune response. Current Opinion in Pharmacology, 9 (4), pp. 514-520.

Perry, M. M., Moschos, S. A., Williams, A. E., Shepherd, N. J., Larner-Svensson, H. M. and Lindsay, M. A., 2008. Rapid changes in microRNA-146a expression negatively regulate the IL-1β-induced inflammatory response in human lung alveolar epithelial cells. The Journal of Immunology, 180 (8), pp. 5689-5698.

Williams, A. E., Perry, M. M., Moschos, S. A., Larner-Svensson, H. M. and Lindsay, M. A., 2008. Role of miRNA-146a in the regulation of the innate immune response and cancer. Biochemical Society Transactions, 36 (6), pp. 1211-1215.

Moschos, S. A., Spinks, K., Williams, A. E. and Lindsay, M. A., 2008. Targeting the lung using siRNA and antisense based oligonucleotides. Current Pharmaceutical Design, 14 (34), pp. 3620-3627.

Lindsay, M. A., 2008. microRNAs and the immune response. Trends in Immunology, 29 (7), pp. 343-351.

This list was generated on Mon Oct 23 07:48:34 2017 IST.

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