You will be expected to visit the lab on four occasions/trial days. You will need to fill out a food diary and refrain from alcohol and physical activity (above your usual routine) for one day prior to a trial day.
On each occasion you will ingest a different test drink (Control, low dose of calcium, medium dose, high dose). Each drink will contain 25g protein, a differing dose of calcium, 500 ml water and low calorie chocolate sweetener.
On a lab day you will need to arrive fasted, having only had water before being fitted with a cannula (a small plastic tube for blood sampling) in the arm for frequent blood samples. Baseline blood samples and visual appetite scales will be completed. You will then ingest the test drink before blood samples at 15, 30, 45, 60, 90, 120 and 180 minutes following the drink. Appetite scales will be filled out hourly.
After 180 minutes, you will be provided with lunch (tomato and cheese pasta) which you will be asked to eat until comfortably full. The whole trial day will last about 4 hours and you will be sat in a bed for the duration, free to watch Netflix, carry out work on your laptop etc.
All data will be kept in a locked cabinet or computerised and accessed only via a password, which will be done in accordance with the Data Protection Act. Throughout the study participants will be identified on all documents with a unique code instead of their name.
This study has received ethical approval.1