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BB10186: Proteins: purification and characterisation

Follow this link for further information on academic years Academic Year: 2014/5
Further information on owning departmentsOwning Department/School: Department of Biology & Biochemistry
Further information on credits Credits: 6
Further information on unit levels Level: Certificate (FHEQ level 4)
Further information on teaching periods Period: Semester 2
Further information on unit assessment Assessment Summary: EX 90%, MC 10%
Further information on unit assessment Assessment Detail:
  • Examination (EX 90%)
  • Multiple Choice (MC 10%)
Further information on supplementary assessment Supplementary Assessment: Like-for-like reassessment (where allowed by programme regulations)
Further information on requisites Requisites: Before taking this unit you must take BB10003 and take CH10088 and while taking this unit you must take BB10004 and take CH10087
Further information on descriptions Description: Aims:
To provide understanding of the principles and applications of various methods for the purification and characterisation of proteins.

Learning Outcomes:
After taking this unit the student should be able to:
* Describe common methods for purifying proteins and be able to design a simple protein purification scheme;
* Describe the use of different spectroscopic techniques to probe structural features of proteins;
* Describe physical techniques used to elucidate the function and oligomeric state of proteins.

Learning and studying T/F/A, Written communication T/F/A, Numeracy & computation T/F/A, Laboratory skills T/F, Information technology T/F, Information handling & retrieval T/F/A, Working independently T/F.

Protein separation by column chromatography based on differences in molecular size, charge, hydrophobicity and specific affinity for ligands; electrophoretic methods for the purification and characterisation of proteins; use of techniques (such as fluorescence, UV/CD spectroscopy, dynamic light scattering, mass spectroscopy, ITC and SPR) to probe function and oligomeric state of proteins.
Further information on programme availabilityProgramme availability:

BB10186 is Compulsory on the following programmes:

Department of Biology & Biochemistry
  • USBB-AFB02 : BSc(Hons) Biochemistry (Year 1)
  • USBB-AKB06 : BSc(Hons) Biochemistry with Professional Placement (Year 1)
  • USBB-AFB05 : BSc(Hons) Molecular and Cellular Biology (Year 1)
  • USBB-AKB08 : BSc(Hons) Molecular and Cellular Biology with Professional Placement (Year 1)

* This unit catalogue is applicable for the 2014/15 academic year only. Students continuing their studies into 2015/16 and beyond should not assume that this unit will be available in future years in the format displayed here for 2014/15.
* Programmes and units are subject to change at any time, in accordance with normal University procedures.
* Availability of units will be subject to constraints such as staff availability, minimum and maximum group sizes, and timetabling factors as well as a student's ability to meet any pre-requisite rules.