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BB20025: Practical molecular biology

Follow this link for further information on academic years Academic Year: 2019/0
Further information on owning departmentsOwning Department/School: Department of Biology & Biochemistry
Further information on credits Credits: 6      [equivalent to 12 CATS credits]
Further information on notional study hours Notional Study Hours: 120
Further information on unit levels Level: Intermediate (FHEQ level 5)
Further information on teaching periods Period:
Semester 2
Further information on unit assessment Assessment Summary: CW 100%
Further information on unit assessment Assessment Detail:
  • Practical Booklet (CW 85%)
  • Demonstration Analysis (CW 15%)
Further information on supplementary assessment Supplementary Assessment:
BB20025 Coursework (where allowed by programme regulations)
Further information on requisites Requisites:
Further information on descriptions Description: Aims:
To provide practical experience of some commonly used molecular biology techniques and to evaluate these in comparison with other available methods so that the students are aware of a range of techniques used for the characterisation of gene structure and gene expression.

Learning Outcomes:
After taking this course the student should be able to:
* explain how and why these techniques have been applied when they encounter them in journal articles
* select appropriate methods of this type when they design their own experiments
* planning and organise carrying out a scientific project
* handle and interpret data of this type generated by these techniques

Learning and studying T/F/A, Written communication T/F/A, Oral communication T/F/A, Numeracy & computation T/F/A, Laboratory skills T/F, Information technology T/F, Problem solving T/F, Information handling & retrieval T/F/A, Working independently T/F, Career preparation T/F.

The course involves a series of linked experiments that form a mini-project. This begins with the preparation of cellular RNA, its conversion to cDNA and the amplification of gene fragments using the polymerase chain reaction (PCR). This is followed by cloning and sequencing. Data interpretation involves DNA sequence analysis using genome databases. A demonstration of various methods for analysing gene expression is included.
Further information on programme availabilityProgramme availability:

BB20025 is Compulsory on the following programmes:

Department of Biology & Biochemistry

BB20025 is Optional on the following programmes:

Department of Biology & Biochemistry