BB20025: Practical molecular biology
[Page last updated: 15 October 2020]
Academic Year: | 2020/1 |
Owning Department/School: | Department of Biology & Biochemistry |
Credits: | 6 [equivalent to 12 CATS credits] |
Notional Study Hours: | 120 |
Level: | Intermediate (FHEQ level 5) |
Period: |
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Assessment Summary: | CW 100% |
Assessment Detail: |
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Supplementary Assessment: |
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Description: | Aims: To provide practical experience of some commonly used molecular biology techniques and to evaluate these in comparison with other available methods so that the students are aware of a range of techniques used for the characterisation of gene structure and gene expression. Learning Outcomes: After taking this course the student should be able to: * explain how and why these techniques have been applied when they encounter them in journal articles * select appropriate methods of this type when they design their own experiments * planning and organise carrying out a scientific project * handle and interpret data of this type generated by these techniques Skills: Learning and studying T/F/A, Written communication T/F/A, Oral communication T/F/A, Numeracy & computation T/F/A, Laboratory skills T/F, Information technology T/F, Problem solving T/F, Information handling & retrieval T/F/A, Working independently T/F, Career preparation T/F. Content: The course involves a series of linked experiments that form a mini-project. This begins with the preparation of cellular RNA, its conversion to cDNA and the amplification of gene fragments using the polymerase chain reaction (PCR). This is followed by cloning and sequencing. Data interpretation involves DNA sequence analysis using genome databases. A demonstration of various methods for analysing gene expression is included. |
Programme availability: |
BB20025 is Compulsory on the following programmes:Department of Biology & Biochemistry
BB20025 is Optional on the following programmes:Department of Biology & Biochemistry
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