The Bio-Imaging and Cell Analysis suite, provides users with a variety of different approaches to image their samples. Services provided include Confocal microscopy, FACs analysis and sorting, High Content Imaging and Analysis, and an advanced Hypoxic Imaging Facility. Training and assistance are provided at all levels, including guidance on planning your imaging experiments and preparing your samples, and help with image/data analysis.

Our bio-imaging instruments are employed in a variety of different projects, including studies of inflammatory diseases, cancer research, stem cell biology, diabetes, neuroscience, developmental biology, and drug discovery.

Confocal laser scanning microscopy

The Confocal laser scanning microscopes enable researchers to create detailed 3D pictures of cell organelles and to examine ‘live’ cells through incubation systems that facilitate the study of cellular changes over time.

  • LSM510META
  • LSM880 with Airyscan and Multiphoton laser

Read on to learn about Second Harmonic Generation microscopy.

Flow cytometry

  • Becton Dickinson flow cytometer FACSCanto
  • Becton Dickinson flow cytometer FACSARIA III

High content imaging and analysis (HCA)

HCA combines cell biology with automated high-resolution microscopy and data evaluation. It is used for the detailed study of cellular mechanisms and interactions in multiple samples, e.g. studying whether cell division, motility, or protein entry is affected when cells are exposed to external stimuli such as chemical inhibitors.

Information generated using HCA is highly valuable in the study of complex diseases, including cancer and infectious diseases. In drug discovery research, the throughput gained using HCA makes it a valuable technology for drug target identification.

GE Healthcare IN Cell Analyzer 2000

Advanced hypoxic imaging facility

Our hypoxic imaging facility consists of three state-of-the-art analytical instruments to study in vitro cell function under hypoxic conditions. Each instrument will measure different aspects of cell or molecular function under continuous hypoxic conditions. The facility includes a Leica Dmi8 Imaging Microscope, and a BMG Labtech CLARIOstar plate reader with regulated oxygen control.

With this facility we can control oxygen levels to study different aspects of cell function, from a single cell through to entire cell populations. This will provide cell models that mimic oxygen levels in living systems. We use these cell models to explore the effects of oxygen deprivation, which can lead to tissue damage and disease.

  • Baker Ruskinn Sci-tive Hypoxic workstation housing an advanced Leica Dmi8 imaging microscope
  • BMG Labtech CLARIOstar plate reader with regulated gas control