Department of Biology & Biochemistry, Unit Catalogue 2009/10 |
BB20021: Protein purification |
 Credits:
| 6 |
| Level:
| Intermediate |
| Period: | Semester 2 |
| Assessment:
| EX 80%, PR 20% |
| Supplementary Assessment: | Like-for-like reassessment (where allowed by programme regulations) |
| Requisites:
| Before taking this unit you must take BB10003 and take BB10004 and take CH10087 and take CH10088 and in taking this unit you cannot take BB20099 |
| Description: | Aims: To provide understanding of the principles and application of the various methods for the purification and characterisation of proteins. To provide understanding of the principles and application of the ultracentrifuge in the biochemistry laboratory. Learning Outcomes: After taking this course the student should be able to: * plan a purification scheme for the isolation of a protein from various starting materials, taking account of requirements for both purity and yield of the product; * design experiments that involve the use of ultracentrifugation techniques. Skills: Learning and studying T/F/A, Written communication T/F/A, Numeracy & computation T/F/A, Laboratory skills T/F, Information technology T/F, Information handling & retrieval T/F/A, Working independently T/F. Content: Protein separation by column chromatography based on differences in molecular size, charge, hydrophobicity and specific affinity for ligands; electrophoretic methods for the purification and characterisation of proteins; theory and practice of preparative and analytical ultracentrifugation. |