Aims & Learning Objectives:
Aims: To provide understanding of the principles and application of the various methods for the purification and characterisation of proteins. To provide understanding of the principles and application of the ultracentrifuge in the biochemistry laboratory.
After taking this course the student should be able to:
* plan a purification scheme for the isolation of a protein from various starting materials, taking account of requirements for both purity and yield of the product
* design experiments that involve the use of ultracentrifugation techniques.
Content:
Protein separation by column chromatography based on differences in molecular size, charge, hydrophobicity and specific affinity for ligands; electrophoretic methods for the purification and characterisation of proteins; theory and practise of preparative and analytical ultracentrifugation
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