The system offers single or two-photon excitation, spectral emission detection, multi-photon imaging, and super resolution with an Airyscan detector.
The microscope is equipped with seven excitation laser lines:
- 405 nanometres
- 458 nanometres
- 488 nanometres
- 514 nanometres
- 561 nanometres
- 594 nanometres
- 633 nanometres
The Zeiss LSM 880 is also equipped with an InSight DS+ Ti:Sapphire femtosecond pulsed laser (tunable between 690 and 1,300 nanometres), being able to excite the vast majority of the commercially available fluorophores.
Depending on the application, emission is detected internally by a 34-channel detector array or the Super Resolution Airyscan detector, and externally by two PMTs at the transmitted path or a BIG.2 detector at the reflected path.
A variety of air, oil, and water objectives offer flexibility in imaging diverse samples. The offered imaging applications cover the biological, physical and material sciences including, but not limited to:
- multi-colour confocal imaging
- multi-colour 3D imaging
- 4D imaging (x, y, z, and time)
- live cell imaging
- multi-photon (two-photon) imaging
- Second/Third Harmonic Generation
- steady-state Förster/fluorescence resonance energy transfer (FRET)
- fluorescence recovery after photobleaching (FRAP)
- spectral unmixing and fingerprinting
- co-localisation studies
- calcium imaging or other ratio imaging