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Zeiss LSM880 Multiphoton Confocal Laser Scanning Fluorescence Microscope

Learn about our Zeiss LSM880 Multiphoton Confocal Laser Scanning Microscope, hosted in the core BioImaging and Cell Analysis Unit within the Imaging Facility.


Neuron in orthogonal projection
Neuron in orthogonal projection

The Zeiss LSM-880 is a fully motorized, multi-modal inverted microscope system, based on a Carl Zeiss Axio Observer.Z1. The system offers single or two-photon excitation, spectral emission detection, multi-photon imaging, super resolution with an Airyscan detector, and full environmental control for Live Cell Imaging. The microscope is equipped with 7 excitation laser lines (405nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm), as well as with an InSight DS+ Ti:Sapphire femtosecond pulsed laser (tunable between 690-1300nm), being able to excite the vast majority of the commercially available fluorophores. Depending on the application, emission is detected internally by a 34-channel detector array or the Super Resolution Airyscan detector, and externally by 2 PMTs at the transmitted path or a BIG.2 detector at the reflected path. A variety of air, oil and water objectives offer flexibility in imaging diverse samples. The offered imaging applications cover the biological, physical and material sciences including, but not limited to, multi-colour confocal imaging, multi-colour 3D imaging, 4D imaging (x, y, z, and time), live cell imaging, multi-photon (two-photon) imaging, Second/Third Harmonic Generation, steady-state Förster/fluorescence resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), spectral unmixing and fingerprinting, co-localisation studies, calcium imaging or other ratio imaging.

Funding details: Application of bioimaging and fluorescence-based bioprobes to study molecular and cellular mechanisms in integrated physiological settings. Wellcome Trust (2005) REF: 077454. PI: Prof. Stephen Ward.


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