Department of Biology & Biochemistry, Unit Catalogue 2010/11 |
BB10186: Proteins: purification and characterisation |
 Credits:
| 6 |
| Level:
| Certificate |
| Period: | This unit is available in... |
| Semester 2 |
| Assessment:
| CW20EX80 |
| Supplementary Assessment: | Supplementary assessment information not currently available (this will be added shortly) |
| Requisites:
| Before taking this unit you must take BB10003 and take CH10088 and while taking this unit you must take BB10004 and take CH10087 |
| Description:
| Aims: To provide understanding of the principles and applications of various methods for the purification and characterisation of proteins. Learning Outcomes: After taking this unit the student should be able to: * Describe common methods for purifying proteins and be able to design a simple protein purification scheme; * Describe the use of different spectroscopic techniques to probe structural features of proteins; * Describe physical techniques used to elucidate the function and oligomeric state of proteins. Skills: Learning and studying T/F/A, Written communication T/F/A, Numeracy & computation T/F/A, Laboratory skills T/F, Information technology T/F, Information handling & retrieval T/F/A, Working independently T/F. Content: Protein separation by column chromatography based on differences in molecular size, charge, hydrophobicity and specific affinity for ligands; electrophoretic methods for the purification and characterisation of proteins; use of techniques (such as fluorescence, UV/CD spectroscopy, dynamic light scattering, mass spectroscopy, ITC and SPR) to probe function and oligomeric state of proteins. |