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BB20144: Techniques in cell biology

Follow this link for further information on academic years Academic Year: 2018/9
Further information on owning departmentsOwning Department/School: Department of Biology & Biochemistry
Further information on credits Credits: 6      [equivalent to 12 CATS credits]
Further information on notional study hours Notional Study Hours: 120
Further information on unit levels Level: Intermediate (FHEQ level 5)
Further information on teaching periods Period:
Semester 1
Further information on unit assessment Assessment Summary: EX 70%, PR 30%
Further information on unit assessment Assessment Detail:
  • Practical (PR 30%)
  • Examination (EX 70%)
Further information on supplementary assessment Supplementary Assessment:
Like-for-like reassessment (where allowed by programme regulations)
Further information on requisites Requisites:
Before taking this module you must take BB10005
While taking this module you must take BB20024
Further information on descriptions Description: Aims:
To give an overview of some of the commonly used techniques in cell biology and to provide an understanding of how these techniques are being applied to address current cell biological problems.

Learning Outcomes:
After taking this course the student should be able to:
* Describe and explain the basic principles involved in each technique;
* Determine and explain when a technique should be used;
* Assess the strengths and weaknesses of each technique;
* Plan their time during the course of an experiment for maximal efficiency and optimal outcome.

Skills:
Learning and studying T/F/A, Written communication T/F/A, Laboratory skills T/F, Information technology T/F, Problem solving T/F, Information handling & retrieval T/F/A, Working independently T/F, Career preparation T/F.

Content:
Lectures will cover the basic principles underlying the techniques of light and electron microscopy including immunofluorescence and confocal microscopy, immunoblotting, immunoprecipitation, cell culture, transfection of mammalian cell lines including considerations of types of promoters and cell type specific expression, subcellular fractionation and metabolic labelling. The practical content will include projects focused on transfection of mammalian cell lines, immunofluroescence, immunoblottting, and subcellular fractionation.
Further information on programme availabilityProgramme availability:

BB20144 is Compulsory on the following programmes:

Department of Biology & Biochemistry
  • USBB-AFB05 : BSc(Hons) Molecular and Cellular Biology (Year 2)
  • USBB-AKB08 : BSc(Hons) Molecular and Cellular Biology with Professional Placement (Year 2)

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