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BB10186: Proteins: purification and characterisation

[Page last updated: 20 April 2021]

Academic Year: 2021/2
Owning Department/School: Department of Biology & Biochemistry
Credits: 6      [equivalent to 12 CATS credits]
Notional Study Hours: 120
Level: Certificate (FHEQ level 4)
Period:
Semester 2
Assessment Summary: EX 75%, MC 25%
Supplementary Assessment:
Like-for-like reassessment (where allowed by programme regulations)
Requisites: Before taking this module you must take BB10003 AND take CH10088
While taking this module you must take BB10121 AND take CH10087
Aims: To provide understanding of the principles and applications of various methods for the purification and characterisation of proteins.

Learning Outcomes: After taking this unit the student should be able to:
* Describe common methods for purifying proteins and be able to design a simple protein purification scheme;
* Describe the use of different spectroscopic techniques to probe structural features of proteins;
* Describe physical techniques used to elucidate the function and oligomeric state of proteins.

Skills: Learning and studying T/F/A, Written communication T/F/A, Numeracy & computation T/F/A, Laboratory skills T/F, Information technology T/F, Information handling & retrieval T/F/A, Working independently T/F.

Content: Protein separation by column chromatography based on differences in molecular size, charge, hydrophobicity and specific affinity for ligands; electrophoretic methods for the purification and characterisation of proteins; use of techniques (such as fluorescence, UV/CD spectroscopy, dynamic light scattering, mass spectroscopy, ITC and SPR) to probe function and oligomeric state of proteins.

Programme availability:
NB. Postgraduate programme information will be added when the postgraduate catalogues are published in August 2021

BB10186 is Compulsory on the following programmes:

Department of Biology & Biochemistry
  • USBB-AFB02 : BSc(Hons) Biochemistry (Year 1)
  • USBB-AKB06 : BSc(Hons) Biochemistry with Professional Placement (Year 1)
  • USBB-AFM06 : MBiochem(Hons) Biochemistry (Year 1)
  • USBB-AKM06 : MBiochem(Hons) Biochemistry with Professional Placement (Year 1)

Notes:

  • This unit catalogue is applicable for the 2021/22 academic year only. Students continuing their studies into 2022/23 and beyond should not assume that this unit will be available in future years in the format displayed here for 2021/22.
  • Programmes and units are subject to change in accordance with normal University procedures.
  • Availability of units will be subject to constraints such as staff availability, minimum and maximum group sizes, and timetabling factors as well as a student's ability to meet any pre-requisite rules.
  • Find out more about these and other important University terms and conditions here.