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CH20195: Analytical chemistry

[Page last updated: 20 April 2021]

Academic Year: 2021/2
Owning Department/School: Department of Chemistry
Credits: 6      [equivalent to 12 CATS credits]
Notional Study Hours: 120
Level: Intermediate (FHEQ level 5)
Period:
Semester 1
Assessment Summary: EX 100%
Supplementary Assessment:
Like-for-like reassessment (where allowed by programme regulations)
Requisites:
Aims: To introduce students to the theoretical basis of analytical chemistry and recent developments in analytical techniques.

Learning Outcomes: After successfully completing this unit, students should be able to:
1. Understand the theoretical basis of analytical techniques including immunoassays, chromatography, mass spectrometry and related techniques.
2. Be aware of current developments in the field of analytical chemistry.
3. Apply this knowledge to the solution of problems relating to qualitative and quantitative analysis.

Skills: Numeracy (F), Problem solving (F, T, A), Independent working (F).

Content: Sample preparation, separation techniques including gas and liquid chromatography, mass spectrometry, coupled techniques (GC-MS and LC-MS), point-of-care analysis (immunoassays), method validation and data handling
* An introduction to separation science.
* Introduction to sample preparation techniques (including solid phase extraction, solid phase micro-extraction, accelerated solvent extraction and derivatisation).
* Principles of solid phase extraction.
* Application of solid-phase extraction in environmental, forensic and pharmaceutical science.
* Theory of chromatography (including plate and rate theory, band broadening, peak asymmetry and separation efficiency).
* Principles of gas and liquid chromatography (factors affecting separation of analytes, types of stationary and mobile phases and detectors).
* Advances in chromatographic techniques: fast chromatography, multidimensional chromatography and chiral chromatography.
* Description of modern mass spectrometry and how a mass spectrometer produces, separates analyses and detects ions.
* Modern ionization techniques including electrospray, cryospray, proton-transfer reaction mass spectrometry.
* Advantages and disadvantages of modern mass spectrometer analyser types including quadrupole, time-of-flight and ion trap.
* Hyphenated techniques: coupling gas and liquid chromatography with mass spectrometry.
* Sensitivity, selectivity, range, limit of detection: definition and determination from analytical data.
* Immunoassays, including AIDS and pregnancy test kit as examples
* Improving analytical signals: signal amplification
* Applications of chromatographic techniques in environmental, forensic and pharmaceutical science.
* Techniques used for protein purification - Fractionation of cell, Ion exchange chromatography, Gel Electrophoresis, Capillary electrophoresis, Size exclusion chromatography, Affinity chromatography, Different assays used to target biological activity.
2) Analysis of sequence of biopolymers - Proteins - (Edman degredation, modern MS techniques), DNA (Traditional Sanger approach, new techniques, automation) and Sugars (old techniques - acylation, tandem ms).
3) Process Analysis of Drugs - Near infrared spectroscopy, Impurity profiling in pharmaceuticals, Process analytical for cGMP, Analysis of crystalline forms - polymorphism, Detecting counterfeit drugs.
4) Drug Discovery - Isolation/structural determination of natural products, brief recap of range of analytical techniques required to characterise drug molecules, analysis of drug-protein interactions, metabolite detection, lab on a chip.

Programme availability:
NB. Postgraduate programme information will be added when the postgraduate catalogues are published in August 2021

CH20195 is Compulsory on the following programmes:

Department of Chemistry
  • USCH-AFB01 : BSc(Hons) Chemistry (Year 2)
  • USCH-AAB02 : BSc(Hons) Chemistry with Study year abroad (Year 2)
  • USCH-AFB07 : BSc(Hons) Chemistry for Drug Discovery (Year 2)
  • USCH-AAB08 : BSc(Hons) Chemistry for Drug Discovery with Study year abroad (Year 2)
  • USCH-AKB08 : BSc(Hons) Chemistry for Drug Discovery with Industrial Placement (Year 2)
  • USCH-AKB02 : BSc(Hons) Chemistry with Industrial Placement (Year 2)
  • USCH-AFM02 : MChem(Hons) Chemistry (Year 2)
  • USCH-AAM03 : MChem(Hons) Chemistry with Study year abroad (Year 2)
  • USCH-AFM05 : MChem(Hons) Chemistry for Drug Discovery (Year 2)
  • USCH-AAM06 : MChem(Hons) Chemistry for Drug Discovery with Study year abroad (Year 2)
  • USCH-AKM03 : MChem(Hons) Chemistry for Drug Discovery with Industrial Placement (Year 2)
  • USCH-AKM02 : MChem(Hons) Chemistry with Industrial Placement (Year 2)

CH20195 is Optional on the following programmes:

Department of Chemistry
  • USCH-AFB03 : BSc(Hons) Chemistry with Management (Year 3)
  • USCH-AAB04 : BSc(Hons) Chemistry with Management with Study year abroad (Year 4)
  • USCH-AKB04 : BSc(Hons) Chemistry with Management with Industrial Placement (Year 4)
  • USCH-AFM07 : MSci(Hons) Chemistry with Management (Year 3)
  • USCH-AKM07 : MSci(Hons) Chemistry with Management with Industrial Placement (Year 4)

Notes:

  • This unit catalogue is applicable for the 2021/22 academic year only. Students continuing their studies into 2022/23 and beyond should not assume that this unit will be available in future years in the format displayed here for 2021/22.
  • Programmes and units are subject to change in accordance with normal University procedures.
  • Availability of units will be subject to constraints such as staff availability, minimum and maximum group sizes, and timetabling factors as well as a student's ability to meet any pre-requisite rules.
  • Find out more about these and other important University terms and conditions here.